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Bloodstream

A1  0.10 Write down the units of the fluid viscosity coefficient $\eta$. Express the answer in the base SI units (kg, m, s).

1 Correct units: $kg/(m \cdot s)$. 0.10
A2  0.90 Using dimensional analysis, find $a$, $b$ and $c$.

1 The following expression is obtained:
$$ L^3 T^{-1} = L^{a} (M L^{-2} T^{-2})^{b} (M L^{-1} T^{-1})^{c}. $$
0.20
2 The correct system of equations is obtained:
$$\begin{cases}
b + c = 0\\
-1 = -2b - c \\
3 = a - 2b - c.
\end{cases}$$
0.10
3 The values are found correctly: $$ a=4,~b=1,~c=-1.$$ 3 × 0.20
B1  0.20 Find the pressure force acting on a cylindrical element of fluid with radius $r$ and length $L$ (see Fig. 2). Express your answer in terms of $\Delta P, r$.

1 Correct expression for the pressure force:
$$F_p = \Delta P \cdot \pi r^2.$$
0.20
B2  0.85 Write down the stationarity condition for flow and find the dependence of $g=\frac{\Delta v}{\Delta r}$ on the radius $r$. Express the answer in terms of $\Delta P, R, r, \eta, L$. Sketch a graph of the dependence $g(r)$.

1 It is indicated that for a stationary flow, the sum of the lengthwise forces acting on the cylindrical element in question is zero:
$$
F_p - F_{fr} = 0.
$$
0.20
2 The expression for the friction force on the lateral surface of a thin layer is obtained:
$$
F_{fr} = \eta\,\frac{-\Delta v}{\Delta r}\cdot 2\pi r L.
$$
0.30
3 Correct expression for $g$:
$$
\frac{-\Delta v}{\Delta r} = -g(r) = \frac{\Delta P}{2\eta L}\, r.
$$
0.20
4 Correct graph of the dependence $g(r)$ is sketched. 0.15
B3  0.60 Find the dependence of the velocity $v(r)$ on the radius $r$. Express the answer in terms of $\Delta P, R, r, \eta, L$.

1 The idea that the change in velocity is proportional to the area under the graph of $g(r)$ was used. 0.20
2 It is obtained that $v \propto (R^2-r^2)$ 0.20
3 Correct expression for $v(r)$:
$$
v(r)=\frac{k}{2}(R^2-r^2)=\frac{\Delta P}{4\eta L}(R^2-r^2).
$$
0.20
B4  0.20 Find the maximum velocity in the center of the tube $v_{\max}$. Express your answer in terms of $\Delta P, R, \eta, L$.

1 Correct expression for the maximal velocity: $$v_{\max} = v(0) = \frac{\Delta P}{4\eta L}\,R^2.$$ 0.20
B5  0.15 Sketch a graph of the dependence of fluid velocity $v$ on squared radius $r^2$.

1 Correct graph of $v(r^2)$ is sketched. 0.15
B6  0.40 Find the total volumetric flow rate $Q$ through a tube with radius $R$. Express your answer in terms of $\Delta P, R, \eta, L$.

1 The area under the graph is computed:
$$
\text{Area} = \frac{v_{\max}\,R^2}{2}.
$$
0.10
2 The total volumetric flow rate is obtained:
$$
Q = \frac{\pi \Delta P R^4}{8\eta L}.
$$
0.30
B7  0.10 Find the expression for the hydrodynamic resistance $Z$ of this tube. Express your answer in terms of $R, \eta, L$.

1 The expression for the hydrodynamic resistance is obtained:
$$Z_{\text{г}} = \frac{8 \eta L}{\pi R^4}.$$
0.10
C1  0.20 What is the fluid flow rate in each of the narrow tubes? Express your answer in terms of $Q$ and $N$.

1 The fluid flow rate in one of the narrow tubes is obtained:
$$Q_1 = \frac{Q}{N}.$$
0.20
C2  0.20 Find by what factor the pressure difference on a wide tube with radius $R$ differs from the pressure difference on one of the parallel narrow tubes. Express your answer in terms of $\alpha, \beta, N$.

1 Correct ratio is obtained:
$$\frac{\Delta P_1}{\Delta P_2} = \frac{\alpha^4N}{\beta}.$$
0.20
D1  0.90 Calculate the hydrodynamic resistances $Z$ of the levels from the table. Round your answers to three significant digits and fill in the table in the answer sheet.

1 The units for hydrodynamic resistances in the table are correct. 0.10
2 Correct values of $Z$ for each level. 4 × 0.20
3 The values of $Z$ are given with an excessive number of significant digits. 4 × -0.10
D2  0.80 Find the pressure difference $\Delta P$ at each level for the volumetric flow rate $Q_0$. Round your answers to three significant digits and fill the table in the answer sheet.

1 Correct values of $\Delta P$ for each level. 4 × 0.20
2 Values of $\Delta P$ are given with an excessive number of significant digits. 4 × -0.10
D3  0.20 By what factor does the resistance of arterioles decrease as their radius increases by $20\%$?

1 It was computed that the resistance of the arterioles decreased by $1.2^4$ times. 0.20
D4  0.80 By what factor does the total pressure difference $\Delta P$ increase to reach the volumetric flow rate $Q$?

1 The new total circuit resistance is computed: $Z_{\rm tot,new} \approx 8.78\times10^7$ Pa$\cdot$s/m${}^3$ 0.20
2 The old total circuit resistance is computed: $Z_{\rm tot,old} \approx 16.6\times10^7$ Pa$\cdot$s/m${}^3$ 0.20
3 The following expression is obtained: $$\frac{\Delta P_{\rm new}}{\Delta P_0}=4\frac{Z_{\rm tot,new}}{Z_{\rm tot,old}}$$ 0.20
4 The correct ratio is computed:
$$\frac{\Delta P_{\rm new}}{\Delta P_0} \approx 2.12.$$
0.20
D5  0.40 By what factor does the mechanical power developed by the heart to pump blood increase compared to when it is at rest?

1 The formula for calculating the mechanical power $N$ is written:
$$N = Q \Delta P.$$
0.30
2 The correct ratio is obtained:
$$\frac{N_{\rm new}}{N_0} \approx 8.5.$$
0.10
E1  0.20

The figure below schematically depicts the circulatory systems of the following vertebrates:

  1. Amphibians;
  2. Mammals;
  3. Fishes;

Match the numbers 1-3 in the list with the letters A-C in the diagram. Each letter can be used only once.


1 3 matches with the answer 1C, 2A, 3B. 0.20
2 1 match with the answer 1C, 2A, 3B. 0.10
E2  0.30 Match the numbers 1-5 on the diagram with the letters A-E. Each letter should be used only once.

1 The number of matches with the answer 1C, 2E, 3A, 4B, 5D (if there are at least two repeated letters, no points are given). 5 × 0.06
E3  0.30 Mark with an "X" the factors that actually help blood flow through the veins.

1 Five matches with the answer A, C, D, F, H (the order may be different), the incorrect letters (B, E, G) are not written out. 0.30
2 At least four correct letters and no more than one incorrect letter. 0.20
3 At least three correct letters and no more than two incorrect letters. 0.10
4 Less than three correct letters or three incorrect letters. 0.00
E4  0.90 The table in the answer sheet contains a list of human hormones. For each hormone, indicate its chemical nature and the organ responsible for its production in the table: write down an "X" in the appropriate cells. In addition, write down an "X" in the last row corresponding to those hormones that cause an increase in arterial pressure (AP).

Chemical nature
2 The number of hormones for which only one cell is marked (in the "Nature" section), and this cell is correct. 6 × 0.05
Organ
4 The number of hormones for which only one cell is marked (in the "Organ" section), and this cell is correct. 6 × 0.05
Increase of the blood pressure
6 $\text{max}(\text{The number of correct answers} - \text{the number of incorrect answers}; 0)$ 3 × 0.10
E5  0.30

Estimate the effective surface area of the lungs $S$.


Use $D = 10^{-11}$ m${}^2$/s, $d = 1$ μm.


1 $W = Q\dfrac{\Delta \nu}{\Delta t}.$
0.10
2 $\Delta p = \Delta n kT.$ 0.10
3 $S\approx 70~\text{m}^2.$ 0.10
F1  0.60 Determine the $\text{pH}$ of the solutions.

1 Correct equation that allows to determine the concentration of $[H^+]$ in solution A, for example: $K_{a_1} = \dfrac{x^2}{0.15 - x}$. 0.20
2 $\text{pH}_A~2.20.$ 0.10
3 Correct equation that allows to determine the concentration of $[\text{H}^+]$ in solution B, for example: $K_{a_1} \approx\dfrac{[\text{H}^+]C_{salt}}{C_{acid}}$. 0.20
4 $\text{pH}_B~3.57.$ 0.10
F2  1.00 Find the new $\text{pH}$ values of the solutions. Calculate the change in $\text{pH}$ in each case.

1 The dilution of solution A is taken into account. 0.10
2 Correct equation that allows to determine the concentration of $[\text{H}^+]$ in solution A, for example: $K_{a_1} = \dfrac{x(0.017 + x)}{0.125 - x} $. 0.10
3 $\text{pH}_A~1.73$ or $1.78$ in assumption $[\text{H}^+] \ll [\text{H}_2\text{CO}_3]$. 0.20
4 $\Delta \text{pH}_A = -0.47.$ 0.05
5 The reaction $\text{NaHCO}_3 + \text{HCl} \rightarrow \text{H}_2\text{CO}_3 + \text{NaCl}$ is taking into account. 0.10
6 Recalculation of the weak acid concentration: $0.142~\text{M}$. 0.10
7 Recalculation of the salt concentration: $0.108~\text{M}$. 0.10
8 Correct equation that allows to determine the concentration of $[H^+]$ in solution B, for example: $[\text{H}^+] \approx \dfrac{K_{a_1} C_{acid}}{C_{salt}}$. 0.10
9 $\text{pH}_B~3.45$. 0.10
10 $\Delta \text{pH}_B = -0.12.$ 0.05
F3  0.20 Find the molarity of carbon dioxide in blood.

1 $[\text{CO}_2] = kp(\text{CO}_2)$. 0.10
2 $[\text{CO}_2] = 1.22\cdot10^{-3}~\text{M}$. 0.10
F4  0.40 Determine the molarity of carbonic acid and the molarity of hydrogencarbonate ions in blood at $\text{pH}~7.4$.

1 $ [\text{H}_2\text{CO}_3]= K_h[\text{CO}_2]$. 0.10
2 $ [\text{H}_2\text{CO}_3] = 3.66\cdot10^{-6}~\text{M}$ 0.10
3 $[\text{HCO}_3^-] = \dfrac{K_{a_1}[\text{H}_2\text{CO}_3]}{[\text{H}^+]} $. 0.10
4 $[\text{HCO}_3^-] = 0.025~\text{M}$. 0.10
F5  0.20 Determine the solubility of carbon dioxide in blood, i.e., the total molarity of all forms of $\text{CO}_2$.

1 $ s = [\text{CO}_2] + [\text{H}_2\text{CO}_3] + [\text{HCO}_3^-] $, or $s \approx [\text{HCO}_3^-]$ and it is noted that concentrations of other forms can be ignored. 0.10
2 $s \approx 0.026~\text{M}$ or $s \approx 0.025~\text{M}$ 0.10
F6  0.40 At $\text{pH}~7.4$, find what fraction $\alpha$ of oxyhemoglobin is protonated. Find the analogous fraction of protonated deoxyhemoglobin $\beta$.

1 $\alpha = \dfrac{[\text{HbO}_2\text{H}]}{[\text{HbO}_2\text{H}] + [\text{HbO}_2^-]}.$ 0.05
2 $[\text{HbO}_2^-] = \dfrac{K_a}{[\text{H}^+]} [\text{HbO}_2\text{H}]$ 0.05
3 $\alpha = 0.86.$ 0.10
4 $\beta = \dfrac{[\text{Hb}\text{H}]}{[\text{Hb}\text{H}] + [\text{Hb}^-]}.$ 0.05
5 $[\text{Hb}^-] = \dfrac{K_a}{[\text{H}^+]} [\text{Hb}\text{H}]$ 0.05
6 $\beta = 0.14$. 0.10
G1  0.30 The gross formula of heme is $\text{C}_a\text{H}_b\text{O}_c\text{N}_d\text{Fe}$, and the mass fractions of the elements in the compound are $w(\text{C}) = 66.24\%$, $w(\text{H}) = 5.23\%$, $w(\text{O}) = 10.38\%$, $w(\text{N}) = 9.09\%$. From this information, determine the numbers $a, b, c$ and $d$.

1 Correct expression that allows to determine $a, b, c, d$ is written. 0.10
2 $a = 34$. 0.05
3 $b = 32$. 0.05
4 $c = 4$. 0.05
5 $d = 4$. 0.05
G2  0.30

Below is a list of chemical bonds that appear in the structures mentioned above. Write down the letters representing the chemical bonds in the appropriate cells of the table below. Note that the same letters may be used in different cells.


A. Disulfid bridges (–S–S–).


B. Ionic bonds.


C. Hydrogen bonds within a molecule.


D. Hydrophobic interactions.


E. Peptide bonds.


F. Intermolecular hydrogen bonds.


1 Only the letter E is indicated for the primary structure. 0.05
2 Only the letter C is indicated for the secondary structure. 0.05
The tertiary structure
4 Only the correct letters A, B, C, D are written. 0.10
5 There are at least three correct letters and no more than one incorrect letter (E or F). 0.05
6 There are less than three correct letters or 2 incorrect letters (E and F). 0.00
The quaternary structure
8 Only the correct letters A, B, D, F are written. 0.10
9 There are at least three correct letters and no more than one incorrect letter (C or E). 0.05
10 There are less than three correct letters or 2 incorrect letters (C and E). 0.00
G3  0.35 The table in the answer sheet lists various proteins found in nature. Indicate which of them, in their functional state, possess a quaternary structure and which only a tertiary structure.

1 The number of correct answers. 7 × 0.05
G4  0.20 Find an expression for $\theta$ in terms of $[\text{L}]$ and $K_a$. What is the equilibrium constant $K_a$ if the ligand concentration at which exactly half of the binding sites are occupied is $[\text{L}]_{0.5}$?

1 $K_a = \dfrac{[\text{PL}]}{[\text{P}][\text{L}]}$ 0.05
2 $\theta = \dfrac{[\text{L}]}{[\text{L}] + 1/K_a}.$ 0.05
3 $K_a = 1 / [\text{L}]_{0.5}$. 0.10
G5  0.25 Sketch graphs showing the dependence of the degree of saturation $\theta$ on the partial pressure of gases. Indicate which graph corresponds to which gas.

1 Both graphs pass through (0,0). 0.05
2 Both graphs have the form of a hyperbola. 0.05
3 Both graphs have a horizontal asymptote of $\theta = 1$. 0.05
4 For carbon monoxide, the graph has plateaued faster than the graph for oxygen. 0.10
G6  0.90 From the given data, determine the standard molar enthalpy of reaction $\Delta_r H^\circ$ for oxygen binding. Plot the required graph on graph paper in the answer sheets.

1 The numerical values of $1/T$ are calculated. 5 × 0.02
2 The axes are labeled and numbered. 0.10
3 The points are correctly plotted on the graph. 5 × 0.03
4 An approximating line was drawn. 0.10
5 The numerical values of $\ln K_a$ were calculated. 5 × 0.02
6 The slope $\alpha$ of the graph $\ln K_a(1/T)$ is determined: $\alpha \approx 6600$ K. 0.20
7 The numerical value of $\Delta H$ is computed: $\Delta H \approx -55$ kJ/mol. 0.15
G7  0.20 Find the dependence of $\log_{10}\left(\dfrac{\theta}{1-\theta}\right)$ on $\log_{10}([\text{L}])$. Express your answer in terms of $n,~\log_{10}([\text{L}])$ and the equilibrium constant $K_a$.

1 $\theta = \dfrac{[\text{L}]^n}{[\text{L}]^n + 1/K_a} .$ 0.10
2 $\log_{10} \left(\dfrac{\theta}{1 - \theta}\right) = n\log_{10}[\text{L}] + \log_{10} K_a$. 0.10
G8  0.20 Determine $n_H$ as the slope of the tangent to the graph at $\theta = 0.5$. What is the theoretical maximum possible value of $n_H$? Let us note that in practice this value is not achieved.

1 $n_H \in [2.4, 3.0]$. 0.10
2 $(n_H)_{max} = 4$. 0.10
G9  0.10

Give the reason why myoglobin cannot be used as an effective oxygen carrier (efective binding and release of oxygen molecules) from the lungs to the tissues.


A. The myoglobin molecule has a hyperbolic oxygen saturation curve.


B. The concentration of myoglobin in the blood is significantly lower than the concentration of hemoglobin.


C. The myoglobin molecule is lighter, which makes it too mobile.


D. The myoglobin molecule is too small, which can cause it to enter other tissues.


1 The answer А. 0.10
G10  0.20

Choose the correct statement:



  1. Curve A corresponds to $\text{pH}~7.2$, curve B corresponds to $\text{pH}~7.6$.

  2. Curve A corresponds to $\text{pH}~7.6$, curve B corresponds to $\text{pH}~7.2$.


1 The answer 2. 0.20
G11  0.10

Which curve corresponds to hemoglobin in the lungs, and which corresponds to hemoglobin in tissues (mark with an "X" in the table in the answer sheets).


1 The table is filled in correctly. 0.10
2 Filling in the table differs from the correct one. 0.00
G12  0.10

In certain conditions, increased level of myoglobin in blood could be a result of:


A. State of alcohol intoxication.


B. Myocardial infarction.


C. Use of sleeping pills.


D. Alzheimer's disease.


1 The answer B. 0.10